Nanoparticles are defined as particulate materials having at least one dimension less than 100 nanometers (nm), even particles could have zero dimension in the case of quantum dots (Bar et al ., 2009). Metal nanoparticles have attracted great interest due to their distinctive characteristics such as catalytic, optical, magnetic and electrical properties (Rassaei et al., 2008). Nanoparticles exhibit a high surface-to-volume ratio with a decrease in particle size. The specific surface area is relevant for catalytic activity and other related properties such as antimicrobial activity (Bae et al., 2010). As the specific surface area of ​​nanoparticles increases, their biological effectiveness will also increase due to the increase in specific surface area (Mukunthan et al., 2011). Noble metal nanoparticles, such as silver, gold and platinum, are widely used in products that come into direct contact with the human body, such as shampoos, soaps, detergents, shoes, cosmetic products and toothpaste, in addition to medical and pharmaceutical applications (Mukunthan et al., 2011).Catharanthus roseus, commonly called Madagascar periwinkle, is a species of Catharanthus native and endemic to Madagascar. Other English names occasionally used include Cape periwinkle, periwinkle rose, periwinkle rose and "old-maid". It is an evergreen subshrub or herbaceous plant up to 1 m tall. The leaves are ovate to oblong, 2.5 to 9 cm long and 1 to 3.5 cm wide, shiny green, glabrous, with a pale midrib and a short petiole 1 to 1.8 cm long; they are arranged in opposite pairs. The flowers are white to dark pink with a darker red center, with a basal tube 2.5 to 3 cm long and a corolla 2 to 5 cm in diameter with five petal-like lobes. The fruit is a pair of fol...... middle of paper ......ion.HT-29 is a colorectal cancer cell. Colorectal cancer was the second most common cancer after breast cancer. It would be the first among men and also the second among women in Peninsular Malaysia. A total of 2,866 cases were registered with the NCR in 2006 and represent 13.2% of all registered cases. The incidence of colorectal cancer in Peninsular Malaysia increases with age, with the overall ASR being 18.4 per 100,000 population. Thirty percent of patients have advanced disease at presentation, either locally or at distant sites. In this context, chemotherapy remains the only viable therapeutic option. However, this option has also been seriously slowed by metastatic colon cancer, which is resistant to many currently used anticancer agents. It is therefore crucial to develop a new potential therapeutic product with the aim of treating cancer..