In the first experiment, our results supported the hypothesis that T. zuluensis responds differently to light exposure. However, its growth was not optimal in the dark. Pathogen growth was significantly different between incubation volumes in light and darkness. Growth under 25% light volume was significantly higher compared to all other light intensities and darkness in each media treatment (Table 5). Our results also showed that there is a significant difference in fungal growth upon exposure to different light intensities. Incubation in different light volumes revealed different diameter growth of T. zuluensis. Growth was lower in darkness as well as in 75% light, but higher in 25% light volume. This indicates that the pathogen requires light but of limited intensity to grow rapidly. Dark incubation showed the lowest growth compared to all light volumes. Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get an original essay However, light preferences vary among different mushrooms, as some mushrooms grow better in darkness and others prefer light conditions. Hatakeyama et al. , 2017 found that light exerted negative effects on the diameter growth of A. oryzae and that conidia production was also repressed during light incubation. Many fungi also require a light pulse to initiate conidia formation, but several other mechanisms are also involved in its regulation. Likewise, different growth stages also regulate conidia initiation and the processes that trigger enzyme production (Tisch and Schmoll, 2010). Biomass production is also another imperative parameter which can be compared from different angles in future research as it is also regulated by light and darkness (Murthy et al., 2015). In their research, the biomass of A. oryzae increased by 11% in the dark compared to the light. Light often persuades or suppresses spore production in fungi and can have varied effects on reproductive development, growth and metabolism (Atoui et al., 2010). In addition to the effects observed on growth by light and darkness, it also affects the nutritional balance of fungi (Carlile, 1965). Substrate availability/utilization for fungi is closely related to sun exposure (Friedl et al., 2008). Light regulation affects not only the utilization but also the absorption of substrates, which ultimately affects the entire metabolic process in the cell because they lack corresponding compounds (Hill, 1976). Limiting these essential compounds inhibits the growth of fungi. (Tisch and Schmoll, 2010) gave the example of glucose uptake in A. ornatus which was significantly inhibited when incubated under light (Hill, 1976), but it induced the formation of conidia. The relationship between glucose and conidia formation was highlighted by the finding (Betina and Zajacova, 1978) that a higher concentration of glucose reduces conidia production, but does not influence growth. In other studies (Graafmans, 1976), light stimulated polysaccharide synthesis in Penicillium isariiforme. The cell wall of fungi is the first place exposed to photons upon illumination (Tisch et..