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Essay / Gene mapping of Sordaria Fimicola - 1513
The main aim of this experiment is to examine the results of crosses of wild-type mutants that influence the arrangement of ascospores in the asci of the fungus Sordaria fimicola. These resulting arrangements help calculate the mapping distance between centromere and spore color genes in Sordaria. My hypothesis was that due to the large number of group observations considered, the data would be underestimated and the results would not fit into the chi-square table. A sample Petri dish containing the two mutant mother cultures is observed after one week. Ascospores must appear in the 2:2:2:2 or 2:4:2 MII pattern for crossover to occur. Then, based on the collected data, the class calculated the distance on the map. If the distance on the map does not match the value obtained by the researchers from the many successful experiments, then the experiment had errors. And for this reason, the class experiment cannot accept the null hypothesis according to the chi-square test. However, our classroom experiment accepted the null hypothesis and hence it was a success.IntroductionThe fungus Sordaria fimicola is commonly used to study different cell cycle processes such as gene assortment and crossing over during meiosis. Given the importance of genetics in today's world, this experience is of crucial value in helping students gain knowledge about the different processes of the cell cycle and learn how to attempt experiments themselves. similar experiences in the future. Sordaria fimicola requires “mitotic and meiotic nuclear divisions to make eight haploid ascospores” (Helm, 1998). This fungus “spends most of its life in a haploid state” (Glase, 1995). When haploid nuclei fuse in cells, they become...... middle of paper ...... crossovers are more common because certain regions of chromosomes have a greater or lesser tendency to form crossovers than others. other regions. . For example, the centromere appears to inhibit crossing over and genes located nearby do not cross over as much as they should, based solely on their physical location. However, because a genetic map is based on crossover frequencies, the relative distances between genes do not correspond to actual physical distances. Works Cited Helms, Doris et al. Biology in the laboratory: third edition. New York: WHFreeman and Company, 1998. Glase, Jon C. A study of genetic linkage and mapping using tetrad analysis in the fungus Sordaria fimicola. New York: Cornell University, 1995.http://www.ableweb.org/volumes/vol-16/1-glase.pdfMitosis and meiosis. (nd). Retrieved from http://kvhs.nbed.nb.ca/gallant/biology/sordaria.html